Composite

Part:BBa_K4194017:Design

Designed by: Jiong Huang   Group: iGEM22_BUCT-China   (2022-09-24)


pRM'-RBS-LacI-LVA-RBS-mRFP1-LVA-terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1032
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1032
    Illegal NotI site found at 1931
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1032
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1032
    Illegal NgoMIV site found at 548
    Illegal AgeI site found at 125
    Illegal AgeI site found at 1813
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization for Escherichia coli has been done.


Source

None

References